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After 4 weeks of oral administration of 5‑amino‑1mq tablets (5–10 mg/kg) to aged mice (18–20 months old), muscle mass was significantly increased, grip strength was enhanced by approximately 40% compared with the control group, and muscle endurance and exercise capacity were also notably improved.Further studies revealed that 5‑amino‑1mq inhibits excessive NNMT activation in muscle tissue, restores NAD⁺ levels, activates the SIRT1 pathway, reduces muscle protein degradation, and promotes muscle protein synthesis, thereby maintaining muscle mass